Compositional, structural and functional cuticle analysis of Prunus laurocerasus L. sheds light on cuticular barrier plasticity

Barrier properties of the hydrophobic plant cuticle depend on its physicochemical composition. The cuticular compounds vary considerably among species but also organs and tissues same throughout developmental stages. As yet, these intraspecific modifications at wax cutin level are only rarely examined. Attempting to further elucidate profiles, we analysed adaxial abaxial surfaces sclerophyllous leaf three stages drupe fruit Prunus laurocerasus, an evergreen model native temperate regions. According gas chromatographic analyses, waxes contained primarily pentacyclic triterpenoids dominated by ursolic acid, whereas biopolyester mainly consisted 9/10,?-dihydroxy hexadecanoic acid. Distinct organ- side-specific patterns were found for lipid loads, compositions carbon chain length distributions. Compositional variations led different structural functional barrier cuticle, which investigated microscopically, infrared spectroscopically gravimetrically. minimum water conductance was highlighted 1 × 10?5 m s?1 perennial, hypostomatous P. laurocerasus 8 few-month-living, stomatous suggesting organ-specific demands.